Application of Bioactive Cotton Packages for Packaging and Storage of Grains Using Aromatic Components

Research | DOI: https://doi.org/10.31579/2768-0487/187

Application of Bioactive Cotton Packages for Packaging and Storage of Grains Using Aromatic Components

  • R. Refaie 1
  • S. Zaghloul 1
  • M.K. Elbisi 1
  • H.A. Shaaban 2*

1Textile Research and Technology Institute.

2Food Industries and Nutrition Research Institute, National Research Centre, 33El-Behoth Street, Dokki, P.O. Box 12622, Giza 12522, Egypt.

*Corresponding Author: Hamdy Abdel -Gawaad Shaaban., Textile Research and Technology Institute, 2 Food Industries and Nutrition Research Institute, National Research Centre, 33El-Behoth Street, Dokki, P.O. Box 12622, Giza 12522, Egypt.

Citation: R. Refaie, S. Zaghloul, M.K. Elbisi and H.A. Shaaba, (2025), Application of Bioactive Cotton Packages for Packaging and Storage of Grains Using Aromatic Components, Journal of Clinical and Laboratory Research, 8(6); DOI:10.31579/2768-0487/187

Copyright: © 2025, H.A. Shaaban. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Received: 24 October 2025 | Accepted: 10 November 2025 | Published: 01 December 2025

Keywords: antimicrobial; bioactive cotton; food package; aromatic components

Abstract

In recent years, Studies into the utility of essential oils and their components in the preservation of food commodities in order to enhance shelf-life has been successfully carried out. These essential oils and their components (Carvacrol, Eugenol and Cinnamaldehyde) can be used as antimicrobials and food preservative agents is of concern because of several reported side effects of synthetic oils. Due to the ability of essential oil constituents to antimicrobial potential it could be used to as a food preservative for grains, cereals, vegetables and fruits. The aim of the treatments were carried out on the treated cotton packaging to maximize the benefit from its antimicrobial activity to extend grain Storage periods. The cotton fabrics treated in three steps: Carboxymethylation of cotton fabric (CMC) Cationization of cotton fabric 3-Treatment of the fabrics by reactive-cyclodextrine (RCD). Modified and unmodified cotton fabrics were treated with antibacterial agent by dipping them at room temperature for 2 hours under stirring in dimethylsulfoxide (DMSO) solution containing 200, 250, 250ppm of euganol, carvacrol and cinnamaldehyde respectively the samples were then roll-squeezed at pick up 100% and dried at room temperature. all treated and untreated fabrics were treated against antimicrobial tests. The types of cotton fabric composite treated by Cinnamaldehyde highest impact on reducing microbial preparation during storage period followed by cotton fabric composite treated by Carvacrol followed by cotton fabric composite treated by Eugenol which is less impactful to reduce microbial count during the storage period.

Introduction

The antimicrobial packaging consider a tool of active packing. Antimicrobial packaging known as a system which can kill or stop pathogenic and spoilage microorganisms which causes contamination of foods. The new trend in antimicrobial packaging is by adding an antimicrobial agents or by using polymers have antimicrobial properties that satisfy traditional packaging requirements. Antimicrobial packaging substances have to increase the lag phase and lessen the rat of growing of microorganisms to develop the shelf life and maintain the quality and safety of foods 1. The natural antimicrobials show sufficient capacity to reduce microbial contamination inside the food package were reported in some study 2,3. Therefore, the adding of efficient antimicrobials to packaging materials food will improve foodstuff shelf life by removing undesirable pathogens and/or delaying microbial spoilage 4,5. Different antimicrobials have been added to different packaging materials. In particular, the bioactive molecules of essential oils and their components, herbs, and spices have been tested due to their, antiviral antifungal, and insecticidal properties 4,6. The carvacrol is one of major components of the Labiatae family, including, Satureja, Origanum, Thymus, Thymbra and Coridothymus7,8, has grow for wide-spectrum antimicrobial activity, and it has been scanned by a large number of researchers worldwide. Carvacrol is consider a monoterpenic phenol. Carvacrol is mentioned to have a big set of biological properties, including antifungal, phytotoxic, insecticidal, antioxidant, antitumor, antimutagenic, antiparasitic, and antimicrobial activities7,9,10. Furthermore, carvacrol in the United States and Europe has been confirmed as a safe food additive due to the “generally recognized as safe” status, and it is also used as a flavoring agent in sweets, chewing gum and beverages 2,11. Based on the presiding reasons, the use of carvacrol in active packaging systems is an emerging area with potential to be applied in the food industry 12,13. Cinnamaldehyde is an effective inhibitor of the development of yeasts, bacteria, and molds as well as toxin production by microorganisms. The inhabitation of the accession of a number of bacteria such as Bacillus spp., Enterobacter sakazakii, Enterobacter spp., E. coli O1587:H7, Escherichia coli, Micrococcus spp., Listeria innocua, and Staphylococcus spp.14,15,16 and postharvest pathogenic molds including Penicillium digitatum. As one of the phenols, eugenol (2-methoxy-4-allylphenol), C10H12O2, is a principal component of clove bud oil (ca. 72-80%). The physical properties of Eugenol is a clove odor, colorless to slightly yellow liquid with a spicy 17. It is also classified as safe material by the FDA. Eugenol appear an inhibitory effect on the growth of B. cereus, Campylobacter jejuni, E. coli, E. coli O1587:H7, P. digitatum, L. monocytogenes Pseudomon fluorescens, Salmonella enterica, S. enteritidis, and S. aureus 18,4. Dorman and Deans 19 reported that eugenol shows the widest spectrum of activity against 24 out of 25 bacteria, except for Leuconostoc cremoris. The aim of the present study was to use types of cotton fabric packaging composite treated by euganol, carvacrol and cinnamaldehyde respectively  to maximize the benefit from its antimicrobial activity to extend grain Storage periods.

Materials and Methods

Materials

Chemicals: Essential oils (Eo) main compounds (Carvocrol, Eugenol and Cinnamaldehyde) used in this study were obtained from sigma-Aldrich and their purity were more than 98%. Monochlorotrizinyl-β-cyclodextrin, referred here as reactive R-cyclodextrin (RCD), was provided by waker chemic GmbH, Germany. Sodium carbonate, sodium hydroxide, acetic acid, monochloro acetic acid were of laboratory grade chemicals. 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (69%) of technical grade chemical was kindly supplied under the commercial name [Quat188] by Aldrich and Dimethyl sulfoxide [DMSO] were obtained from sigma-Aldrich and their purity were than 99%. Fabrics: Cotton fabric mill de-sized, scoured or bleached, plain weave was supplied by Misr Company for spinning and weaving Mehalla El-Kobra, Egypt. The fabric was further purified in the laboratory by washing at 100oC for 60 minutes using a solution containing 2g/L sodium carbonate and nonionic wetting agent (Egyptol). The fabric was then washed several times with boiling water then with running water and finally dried at ambient conditions.

Methods

In the presented study the cotton fabrics treated in three steps: Carboxymethylation of cotton fabric (CMC) Cationization of cotton fabric 3-Treatment of the fabrics by reactive-cyclodextrine (RCD) , Modified and unmodified cotton fabrics were treated with antibacterial agent by dipping them at room temperature for 2 hours under stirring in dimethylsulfoxide (DMSO) solution containing 200, 250, 250ppm of euganol, carvacrol and cinnamaldehyde respectively the samples were then roll-squeezed at pick up 100% and dried at room temperature. All treated and untreated fabrics were treated against antimicrobial tests. 

Cationization of cotton fabric

Chemical modification of the cotton fabric through cationization was carried out as per the pad-dry-cure method20. The experimental procedures adopted were as follows: 3-Chloro-2-hydroxypropyl trimethyl ammonium chloride (Quat-188) was mixed with sodium hydroxide solution at a NaOH/Quat-188 molar ratio of 2:1. The cotton fabric was padded in this mixture in two dips and two nips, and then squeezed to a wet pick-up of about 100%. The cotton fabric was dried at 40oC for 10min and cured at 120oC for 3min. Thus treated cotton gauze was washed with cold water and 1

Testing and analysis

Mechanical properties test:

The mechanical properties were evaluated by strip method according to ASTM D 5035:2006 using a universal testing machine (INSTRON 4201) at room temperature with crosshead speed of 20 mm/min. The tensile strength and elongation at auto break were measured for both untreated cotton fabrics and those treated fabrics. The samples were cut into strips of 5 cm width and 20 cm length, and every data point is the average of three measurements 23.

Antibacterial activity test:

The antibacterial properties of the treated cotton fabrics were evaluated according to an American Association of Textile Chemist and Colorists (AATCC) test method 100−2004 24. Two bacteria, Gram-positive bacteria, Staphylococcus aurous, abbreviated as S. aurous, and Gram-negative bacteria, Escherichia coli, abbreviated as E. coli, were used. A total of 10μg of the treated cotton fabrics was added to a tube containing 5mL of freshly prepared brain heart infusion broth BHIB (HiMedia, India), which is inoculated with the nominated bacteria (1.6×105/mL). The tubes were incubated at 37°C for 24h in the presence of light source. The turbidity of the test tubes was compared visually to the control BHIB tube. Each tube was diluted, and fractions were plated on Nutrient Agar plates and incubated at 37°C for 24h. Colony forming units/mL was calculated by multiplying the number of colonies by the dilution factor. Antibacterial activities were expressed in terms of the percentage reduction of the microorganisms and calculated by eq 1.

Where A and B are the number of microorganism colonies on untreated and treated cotton fabrics, respectively.

Microbial count test         

Microbiological examination: The microbiological examinations of samples included the determination of total aerobic and anaerobic counts, mould counts, Coliform, and Bacillus cereus. Twenty-five grams of each sample were homogenized in 225 mL peptone water (0.1%) using a stomacher model 400 (Seward Laboratory, London, UK) for 1-2 minutes to give a final dilution of 1:10. Samples were then serially diluted and plated using the appropriate medium.

Total bacteria 

Were placed on PCA medium using pour plate technique according to 25 . The inoculated plates were incubated at 35oC for 3 days. The developing colonies were counted, and the Total Aerobic Bacterial counts (TABC) were expressed as colony forming units (CFU) per gram of samples.

Escherichia coli:

Escherichia coli was counted according to the method described by 26 using MCA medium. The plates were incubated at 44oC for 24-48 hr and the suspected colonies were streaked on EMBA and incubated at 44oC for 24 h.   

Total coliform: 

plate count agar was poured into the plates 26, evenly distributed and incubated at 37°C for 48 h. Colonies were counted using an illuminated magnifying colony counter. To evaluate any difference between the use of water and saline, this procedure was repeated using sterile saline.

Bacillus cereus

Bacillus cereus was enumerated on MEYPA 27 . The plates were incubated for 16- 24 h at 37oC. Confirmation tests of suspected colonies were biochemically performed by testing acid formation from different sugars. 

Total mold count 

Per gram sample were counted on CDYEA medium according to 28 using pour plate technique. Samples were serially diluted, plated and the inoculated plates were incubated at 25oC for 3-5 days and then counted.

Results And Discussion

Scoured cotton, CMC and cationized cotton fabric were reacted separately with reactive-cyclodextrin (RCD) by pad-dry-cure method by using an aqueous solution containing RCD (100g/l) and 20g/l sodium hydroxide. Then modified cotton, CMC and cationized fabrics were treated separately with antibacterial agents including Euganol, Carvacrol and Cinnamaldehyde as previously described in the experimental part. Mechanical properties of treated and untreated fabrics were evaluated and all the treated and untreated samples were monitored for antibacterial, and Microbial count test. Results obtained along with appropriate discussion is follows.

Mechanical Properties

Modification of scoured cotton in different conditions for obtaining CMC as well as Cationized fabrics in addition to other treatments with RCD and antimicrobial agents in specific conditions may lead to loss of the tensile strength of the treated fabrics, so mechanical properties of the treated and untreated samples were evaluated due to its importance in storage package properties, Measured properties were the elongation at auto break and tensile strength for the fabrics before and after treatments. 

Table 1 shows the mechanical properties of untreated and treated samples with different treatments with RCD or with RCD followed by different antimicrobial agents (Euganol, Carvacrol and Cinnamaldehyde).

Mechanical PropertiesTreatmentSubstrate No
Elongation at auto break (mm)Tensile strength (KgF)
11.5 ± 381.85 ± 6Scoured CottonBlank
11.0 ± 375.65 ± 5Cotton treated with RCD1
10.8 ± 374.70 ± 5Cotton treated with RCD followed by Cinnamaldhyde2
10.8 ± 373.95 ± 5Cotton treated with RCD followed by Carvacrol3
10.9 ± 374.15 ± 5Cotton treated with RCD followed by Eugenol4
10.6 ± 375.50 ± 4CMC5
10.5 ± 373.75 ± 4CMC treated with CD6
10.6 ± 373.90 ± 4CMC treated with CD followed by Cinnamaldhyde7
10.2 ± 272.65 ± 4CMC treated with CD followed by Carvacrol8
10.5 ± 274.10 ± 4CMC treated with CD followed by Eugenol9
10.25 ± 272.80 ± 4Cationized Cotton10
10.25 ± 272.15 ± 4Cationized Cotton treated with CD11
10.00 ± 271.85 ± 4Cationized Cotton treated with CD followed by Cinnamaldhyde12
9.80± 271.25 ± 4Cationized Cotton treated with CD followed by Carvacrol13
9.80 ± 271.0 ± 4Cationized Cotton treated with CD followed by Eugenol14

Table I: Effect of treatment type on the Mechanical properties of cotton fabrics.

All The values are means of triplicate ±SD

It is observed that the untreated sample has elongation around 11.5mm and tensile strength of 81.85kgF. After treatment with RCD or with RCD followed by different antimicrobial agents (Euganol, Carvacrol and Cinnamaldehyde), followed by drying and curing, the elongation and tensile strength marginally decrease and reach 10.8mm and 73.95kgF, respectively indicating that the treatment causes a loss in the elongation at break by 6% and tensile strength by about 9%, for CMC treated fabrics the elongation and tensile strength marginally decrease and reach 10.2mm and 72.65kgF, respectively indicating that the treatment causes a loss in the elongation at break by 11% and tensile strength by about 11%, for cationized treated fabrics the elongation and tensile strength marginally decrease and reach 9.80mm and 71.0kgF, respectively indicating that the treatment causes a loss in the elongation at break by 15% and tensile strength by about 14% and these losses percentage are practically acceptable for all treated samples and proved that such treatments does not significantly damage the strength properties of cotton 5,14,20.

Antibacterial properties

Antibacterial activates of the treated and untreated cotton fabric were determined against two kind of bacteria, namely Staphylococcus aureus (S. aureus) (as gram-positive bacteria) and Escherichia coli (E. coli) (as gram-negative bacteria) according to Agar Diffusion Method (AATCC Test Method 100-2004) 24. Inhibition zone diameter formed around the test samples were taken as a measure for antimicrobial activity. Results obtained are set out in Table 2.

Inhibition zone (mm)TreatmentSubstrate No
S. aureus (G+ve)E. coli (G-ve)
0.00.0Scoured CottonBlank
2119Cotton treated with RCD1
2826Cotton treated with RCD followed by Cinnamaldhyde2
2625Cotton treated with RCD followed by Carvacrol3
2624Cotton treated with RCD followed by Eugenol4
1715CMC5
2119CMC treated with CD6
2724CMC treated with CD followed by Cinnamaldhyde7
2524CMC treated with CD followed by Carvacrol8
2422CMC treated with CD followed by Eugenol9
2119Cationized Cotton10
2624Cationized Cotton treated with CD11
3029Cationized Cotton treated with CD followed by Cinnamaldhyde12
2826Cationized Cotton treated with CD followed by Carvacrol13
2726Cationized Cotton treated with CD followed by Eugenol14

Table 2: Effect of treatment type on the antibacterial properties of cotton fabrics.

Untreated scoured fabrics did not show any antimicrobial properties towards S. aureus or E. Coli. 

  1. All cotton substrates CMC or cationized cotton shows antimicrobial properties towards S. aureus or E. Coli.
  2. Different treatments of cotton fabrics with RCD or with RCD and oils extract enhances its antibacterial activity towards both S. aureus and E. Coli where the inhibition zone RCD treated cotton fabric shows 19 and 21mm for E. Coli and S. aureus respectively, presence of oils with RCD lead to increase the inhibition zone which increases to 26 and 28mm for Cinnamaldhyde oil and for Carvacrol oil increases to 25 and 26mm while increases with Eugenol oil to 24 and 26 for. E. Coli and S. aureus respectively proving the improvements of antibacterial properties for the treated cotton fabrics.
  3. Carboxymethylated cotton (CMC) show inhibition zone 15, 17 towards E. Coli and  S. aureus respectively,the treatments of (CMC) with RCD or with RCD and oils extract enhances its antibacterial activity towards both S. aureus and E. Coli where the inhibition zone RCD treated CMC fabric shows 19 and 21mm for E. Coli and  S. aureus  respectively, presence of oils with RCD lead to increase the inhibition zone which increases to 24 and 27mm for Cinnamaldhyde oil and for Carvacrol oil increases to 24 and 25mm while increases with Eugenol oil to 22 and 24 for. E. Coli and r S. aureus respectively proving the improvements of antibacterial properties for the treated CMC fabrics.
  4. Cationized cotton show inhibition zone 19, 21 towards E. Coli and  S. aureus respectively,the treatments of cationized cotton with RCD or with RCD and oils extract enhances its antibacterial activity towards both S. aureus and E. Coli where the inhibition zone RCD treated cationized fabric shows 24 and 26mm for E. Coli and  S. aureus  respectively, presence of oils with RCD lead to increase the inhibition zone which increases to 29 and 30mm for Cinnamaldhyde oil and for Carvacrol oil increases to 26 and 28mm while increases with Eugenol oil to 26 and 27 for. E. Coli and r S. aureus respectively proving the improvements of antibacterial properties for the treated cationized fabrics.
  5. All the treated fabrics indicates enhancement in its antibacterial activity towards both S. aureus and E. Coli and the inhibition zone of treated cationzed cotton with RCD followed by cinnamaldhyde is the best between all the treated fabrics. These results obtained similar than those obtained by Hashem et al.,29. 

Results of microbial count test

A known weight from Egyptian wheat were stored in a package made from treated cotton package for 9 months and the microbial count was monitored 0,1,3, 6 and 9 month.  In this report, we will present the data obtained during 9 months. The package content was monitored for Total anaerobic bacteria count, Total mould count, Coliform and Bacillus cereus.  Microbial count results for wheat storage period from one month to nine months in various treatments of cotton fabric which has been treated in the three volatile active compounds (Carvacrol, Eugenol and Cinnamaldehyde) showed that the cotton fabric treatment which named (cationized+cyclodextrin with three volatile active compounds) is the highest impact in reducing microbial count during storage period followed by cotton fabric (CMC+cyclodextrin with three volatile active compounds ) followed by (Cotton+cyclodextrin with three volatile active compounds) which is less impactful to reduce microbial count. The types of cotton fabric composite treated by Cinnamaldehyde highest impact on reducing microbial preparation during storage period followed by cotton fabric composite treated by Carvacrol followed by cotton fabric composite treated by Eugenol which is less impactful to reduce microbial count during the storage period tables (3, 4, and 5). Also, all treatments were the results of counting at zero time. These results can be supported by the finding of Sanla-Ead et al. 30 who reported that the cinnamaldehyde and eugenol had 'moderate−strong inhibitory' and 'strong−highly solid inhibitory' qualities, individually. Additionally, shows the potential utilization of cinnamaldehyde and eugenol for application in antimicrobial bundling film or covering. In this examination cinnamaldehyde and eugenol were explored for their antimicrobial movement against 10 pathogenic and decay microbes and three strains of yeast. Cinnamaldehyde-consolidated and eugenol-joined methyl cellulose films were set up to acquire dynamic antimicrobial bundling materials. Also, (Bnyan et al. 24 showed that the inhibition effect of carvacrol in various grouping of bacterial development were an extraordinary hindrance development on completely considered bacterial segregates aside from Pseudomonas aeroginosa.

TreatmentTime (Month)Total anaerobic bacteria countTotal mould countColiformBacillus cereusTotal bacterial count
All Treatments09 × 1047× 1088 × 1046 × 1059 × 106
Cotton treated with CD19 × 1047 × 1088× 1046× 1059 × 106
39 × 1048× 1088× 1046× 1059 × 106
68 × 1049 × 1087 × 1045 × 1058× 106
98× 1041 × 1097 × 1045 × 1058× 106
Cotton treated with CD followed by Cinnamaldhyde16 × 1045 × 1085× 1044 × 1056× 106
35 × 1044× 1083× 1043 × 1055 × 106
62 × 1042 × 1082 × 1042 × 1053 × 106
91 × 1041 × 1081 × 1041 × 1052 × 106
Cotton treated with CD followed by Carvacrol17 × 1046× 1086 × 1045 × 1057× 106
36 × 1045 × 1084 × 1044 ×1056 × 106
64× 1043 × 1083 × 1043 × 1055 × 106
93 × 1042× 1082× 1042 ×1054 × 106
Cotton treated with CD followed by Eugenol18 × 1047× 1087 × 1046 × 1058 × 106
37 × 1046 × 1085 × 1045 × 1057 × 106
65 × 1044 × 1084 × 1044 × 1056 × 106
93 × 1043 × 1083× 1043 × 1055× 106

Table 3: Results of antimicrobial count test of cotton substrates in which Egyptian Wheat stored for different periods.

TreatmentTime (Month)Total anaerobic bacteria countTotal mould countColiformBacillus cereusTotal bacterial count
All Treatments09 × 1047× 1088 × 1046 × 1059 × 106
Carboxymethylated Cotton (CMC) treated with CD19 × 1048 × 1088× 1046× 1059 × 106
39 × 1049× 1088× 1046× 1059 × 106
68 × 1049 × 1087 × 1045 × 1058× 106
98× 1041 × 1097 × 1045 × 1058× 106
Carboxymethylated Cotton (CMC) treated with CD followed by Cinnamaldhyde15 × 1044× 1084× 1043 × 1055× 106
34× 1043× 1082× 1042 × 1054 × 106
61× 1041× 1081× 1041 × 1052 × 106
99 × 1038 × 1079 × 1038 × 1041 × 106
Carboxymethylated Cotton (CMC) treated with CD followed by Carvacrol16× 1045× 1085 × 1044 × 1056× 106
35× 1044× 1083 × 1043×1055 × 106
63× 1042 × 1082 × 1042 × 1054× 106
92× 1041× 1081× 1041×1053 × 106
Carboxymethylated Cotton (CMC) treated with CD followed by Eugenol17 × 1046× 1086 × 1045 × 1057 × 106
36× 1045 × 1085 × 1044 × 1056 × 106
64× 1043× 1083 × 1043 × 1055× 106
92 × 1042 × 1082× 1042 × 1054× 106

Table 4: Results of antimicrobial count test of Carboxymethylated cotton substrates in which Egyptian Wheat stored for different periods.

TreatmentTime (Month)Total anaerobic bacteria countTotal mould countColiformBacillus cereusTotal bacterial count
All Treatments09 × 1047× 1088 × 1046 × 1059 × 106
Cationized Cotton treated with CD19 × 1048 × 1088× 1046× 1059 × 106
39 × 1049× 1088× 1046× 1059 × 106
68 × 1049 × 1087 × 1045 × 1058× 106
98× 1041 × 1097 × 1045 × 1058× 106
Cationized Cotton treated with CD followed by Cinnamaldhyde11 × 1041× 1082× 1041 × 1053× 106
37× 1036× 1078× 1038 × 1041 × 106
66× 1033× 1075× 1037 × 1046 × 105
93× 1036 × 1061 × 1033 × 1042 × 105
Cationized Cotton treated with CD followed by Carvacrol13× 1043× 1083 × 1043 × 1054× 106
32× 1042× 1082 × 1042×1053 × 106
61× 1041 × 1088 × 1039 × 1041× 106
98× 1038× 1075× 1037×1048 × 105
Cationized Cotton treated with CD followed by Eugenol14 × 1044× 1084 × 1044 × 1056× 106
33× 1043 × 1083× 1043 × 1055 × 106
62× 1042× 1081 × 1042× 1053× 106
91 × 1041× 1089× 1038 × 1041× 106

Table 5: Results of antimicrobial count test of Cationized cotton substrates in which Egyptian Wheat stored for different periods.

Various investigators have used essential oils and their components , either in pure or formulation forms, to enhance the shelf-life of food commodities in different storage containers such as those made of cardboard, tin, glass, polyethylene, or natural fabrics and have observed significant enhancement of shelf-life 20. An earlier study reported that some essential oil constituents such as carvacrol, eugenol, cinnamaldehyde, citronellol, farnesol, and nerol capable of protecting chili fruits and seeds from fungal infection for up to 6 months 21,31. The use of Cymbopogon pendulous essential oil as a fumigant increased groundnut shelf-life by 6–12 months 32, which proves to be more effective than P. roxburghii essential oil. These differences in efficacy of essential oils components may be related to the use of oils from different plant species, as well as to their chemical composition, dose level, and storage container type. Bioactivity may be associated with the presence of high content of cinnamaldehyde in the composition of essential oils. Therefore, these major components may be considered as possible sources for the development of new antimicrobial agents and may be used in synergy with currently available synthetic antibiotics or antimicrobials. In addition, the cinnamaldehyde molecule is promising as a prototype for derivatives with antibacterial properties and extended antibiotics 33. The all results were presented for illustrate and comparison between different treatments and arranged from highest to lowest impact effect on the microbial count in table (6).

TreatmentTime (Month)Total anaerobic bacteria countTotal mould countColiformBacillus cereusTotal bacterial count
All Treatments09 × 1047× 1088 × 1046 × 1059 × 106
Cotton treated with RCD19 × 1047 × 1088× 1046× 1059 × 106
39 × 1048× 1088× 1046× 1059 × 106
68 × 1049 × 1087 × 1045 × 1058× 106
98× 1041 × 1097 × 1045 × 1058× 106
CMC treated with RCD19 × 1048 × 1088× 1046× 1059 × 106
39 × 1049× 1088× 1046× 1059 × 106
68 × 1049 × 1087 × 1045 × 1058× 106
98× 1041 × 1097 × 1045 × 1058× 106
Cationic treated with RCD19 × 1048 × 1088× 1046× 1059 × 106
39 × 1049× 1088× 1046× 1059 × 106
68 × 1049 × 1087 × 1045 × 1058× 106
98× 1041 × 1097 × 1045 × 1058× 106
Cotton treated with RCD followed by Carvacrol17 × 1046× 1086 × 1045 × 1057× 106
36 × 1045 × 1084 × 1044 ×1056 × 106
64× 1043 × 1083 × 1043 × 1055 × 106
93 × 1042× 1082× 1042 ×1054 × 106
CMC treated with RCD followed by Carvacrol16× 1045× 1085 × 1044 × 1056× 106
35× 1044× 1083 × 1043×1055 × 106
63× 1042 × 1082 × 1042 × 1054× 106
92× 1041× 1081× 1041×1053 × 106
Cationized Cotton treated with RCD followed by Carvacrol13× 1043× 1083 × 1043 × 1054× 106
32× 1042× 1082 × 1042×1053 × 106
61× 1041 × 1088 × 1039 × 1041× 106
98× 1038× 1075× 1037×1048 × 105
TreatmentTime (Month)Total anaerobic bacteria countTotal mould countColiformBacillus cereusTotal bacterial count
All Treatments09 × 1047× 1088 × 1046 × 1059 × 106
Cotton treated with RCD followed by Eugenol18 × 1047× 1087 × 1046 × 1058 × 106
37 × 1046 × 1085 × 1045 × 1057 × 106
65 × 1044 × 1084 × 1044 × 1056 × 106
93 × 1043 × 1083× 1043 × 1055× 106
CMC treated with RCD followed by Eugenol17 × 1046× 1086 × 1045 × 1057 × 106
36× 1045 × 1085 × 1044 × 1056 × 106
64× 1043× 1083 × 1043 × 1055× 106
92 × 1042 × 1082× 1042 × 1054× 106
Cationic fabric treated with RCD followed by Eugenol14 × 1044× 1084 × 1044 × 1056× 106
33× 1043 × 1083× 1043 × 1055 × 106
62× 1042× 1081 × 1042× 1053× 106
91 × 1041× 1089× 1038 × 1041× 106
Cotton treated with CD followed by Cinnamaldhyde16 × 1045 × 1085× 1044 × 1056× 106
35 × 1044× 1083× 1043 × 1055 × 106
62 × 1042 × 1082 × 1042 × 1053 × 106
91 × 1041 × 1081 × 1041 × 1052 × 106
CMC treated with RCD followed by Cinnamaldhyde oil15 × 1044× 1084× 1043 × 1055× 106
34× 1043× 1082× 1042 × 1054 × 106
61× 1041× 1081× 1041 × 1052 × 106
99 × 1038 × 1079 × 1038 × 1041 × 106
Cationized Cotton treated with RCD followed by Cinnamaldhyde11 × 1041× 1082× 1041 × 1053× 106
37× 1036× 1078× 1038 × 1041 × 106
66× 1033× 1075× 1037 × 1046 × 105
93× 1036 × 1061 × 1033 × 1042 × 105

Table 6: Comparison between all substrates in the presence of RCD and RCD+Carvacrol, Eugenol and Cinnamaldhyde.

Conclusion

Carvacrol, Eugenol and Cinnamaldehyde as essential oil constituents have pronounced antimicrobial and food preservative properties that have great significance in the food industry. Along these lines, the different properties of essential oil constituents offer the chance of utilizing normal, safe, eco-accommodating, practical, inexhaustible, and effectively biodegradable antimicrobials for food product protection sooner rather than later. The types of cotton fabric composite treated by Cinnamaldehyde highest impact on reducing microbial preparation during storage period followed by cotton fabric composite treated by Carvacrol followed by cotton fabric composite treated by Eugenol which is less impactful to reduce microbial count during the storage period.

Competing Interests: The authors declare that they have no competing interests. 

Authors’ Contributions: All authors contributed equally to the conception and design of the study. All authors have read and agreed to the published version of the manuscript. 

References

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Mina Sherif Soliman Georgy

We would like to thank the Journal of Thoracic Disease and Cardiothoracic Surgery because of the services they provided us for our articles. The peer-review process was done in a very excellent time manner, and the opinions of the reviewers helped us to improve our manuscript further. The editorial office had an outstanding correspondence with us and guided us in many ways. During a hard time of the pandemic that is affecting every one of us tremendously, the editorial office helped us make everything easier for publishing scientific work. Hope for a more scientific relationship with your Journal.

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Layla Shojaie

The peer-review process which consisted high quality queries on the paper. I did answer six reviewers’ questions and comments before the paper was accepted. The support from the editorial office is excellent.

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Sing-yung Wu

Journal of Neuroscience and Neurological Surgery. I had the experience of publishing a research article recently. The whole process was simple from submission to publication. The reviewers made specific and valuable recommendations and corrections that improved the quality of my publication. I strongly recommend this Journal.

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Orlando Villarreal

Dr. Katarzyna Byczkowska My testimonial covering: "The peer review process is quick and effective. The support from the editorial office is very professional and friendly. Quality of the Clinical Cardiology and Cardiovascular Interventions is scientific and publishes ground-breaking research on cardiology that is useful for other professionals in the field.

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Katarzyna Byczkowska

Thank you most sincerely, with regard to the support you have given in relation to the reviewing process and the processing of my article entitled "Large Cell Neuroendocrine Carcinoma of The Prostate Gland: A Review and Update" for publication in your esteemed Journal, Journal of Cancer Research and Cellular Therapeutics". The editorial team has been very supportive.

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Anthony Kodzo-Grey Venyo

Testimony of Journal of Clinical Otorhinolaryngology: work with your Reviews has been a educational and constructive experience. The editorial office were very helpful and supportive. It was a pleasure to contribute to your Journal.

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Pedro Marques Gomes

Dr. Bernard Terkimbi Utoo, I am happy to publish my scientific work in Journal of Women Health Care and Issues (JWHCI). The manuscript submission was seamless and peer review process was top notch. I was amazed that 4 reviewers worked on the manuscript which made it a highly technical, standard and excellent quality paper. I appreciate the format and consideration for the APC as well as the speed of publication. It is my pleasure to continue with this scientific relationship with the esteem JWHCI.

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Bernard Terkimbi Utoo

This is an acknowledgment for peer reviewers, editorial board of Journal of Clinical Research and Reports. They show a lot of consideration for us as publishers for our research article “Evaluation of the different factors associated with side effects of COVID-19 vaccination on medical students, Mutah university, Al-Karak, Jordan”, in a very professional and easy way. This journal is one of outstanding medical journal.

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Prof Sherif W Mansour

Dear Hao Jiang, to Journal of Nutrition and Food Processing We greatly appreciate the efficient, professional and rapid processing of our paper by your team. If there is anything else we should do, please do not hesitate to let us know. On behalf of my co-authors, we would like to express our great appreciation to editor and reviewers.

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Hao Jiang

As an author who has recently published in the journal "Brain and Neurological Disorders". I am delighted to provide a testimonial on the peer review process, editorial office support, and the overall quality of the journal. The peer review process at Brain and Neurological Disorders is rigorous and meticulous, ensuring that only high-quality, evidence-based research is published. The reviewers are experts in their fields, and their comments and suggestions were constructive and helped improve the quality of my manuscript. The review process was timely and efficient, with clear communication from the editorial office at each stage. The support from the editorial office was exceptional throughout the entire process. The editorial staff was responsive, professional, and always willing to help. They provided valuable guidance on formatting, structure, and ethical considerations, making the submission process seamless. Moreover, they kept me informed about the status of my manuscript and provided timely updates, which made the process less stressful. The journal Brain and Neurological Disorders is of the highest quality, with a strong focus on publishing cutting-edge research in the field of neurology. The articles published in this journal are well-researched, rigorously peer-reviewed, and written by experts in the field. The journal maintains high standards, ensuring that readers are provided with the most up-to-date and reliable information on brain and neurological disorders. In conclusion, I had a wonderful experience publishing in Brain and Neurological Disorders. The peer review process was thorough, the editorial office provided exceptional support, and the journal's quality is second to none. I would highly recommend this journal to any researcher working in the field of neurology and brain disorders.

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Dr Shiming Tang

Dear Agrippa Hilda, Journal of Neuroscience and Neurological Surgery, Editorial Coordinator, I trust this message finds you well. I want to extend my appreciation for considering my article for publication in your esteemed journal. I am pleased to provide a testimonial regarding the peer review process and the support received from your editorial office. The peer review process for my paper was carried out in a highly professional and thorough manner. The feedback and comments provided by the authors were constructive and very useful in improving the quality of the manuscript. This rigorous assessment process undoubtedly contributes to the high standards maintained by your journal.

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Raed Mualem

International Journal of Clinical Case Reports and Reviews. I strongly recommend to consider submitting your work to this high-quality journal. The support and availability of the Editorial staff is outstanding and the review process was both efficient and rigorous.

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Andreas Filippaios

Thank you very much for publishing my Research Article titled “Comparing Treatment Outcome Of Allergic Rhinitis Patients After Using Fluticasone Nasal Spray And Nasal Douching" in the Journal of Clinical Otorhinolaryngology. As Medical Professionals we are immensely benefited from study of various informative Articles and Papers published in this high quality Journal. I look forward to enriching my knowledge by regular study of the Journal and contribute my future work in the field of ENT through the Journal for use by the medical fraternity. The support from the Editorial office was excellent and very prompt. I also welcome the comments received from the readers of my Research Article.

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Dr Suramya Dhamija

Dear Erica Kelsey, Editorial Coordinator of Cancer Research and Cellular Therapeutics Our team is very satisfied with the processing of our paper by your journal. That was fast, efficient, rigorous, but without unnecessary complications. We appreciated the very short time between the submission of the paper and its publication on line on your site.

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Bruno Chauffert

I am very glad to say that the peer review process is very successful and fast and support from the Editorial Office. Therefore, I would like to continue our scientific relationship for a long time. And I especially thank you for your kindly attention towards my article. Have a good day!

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Baheci Selen

"We recently published an article entitled “Influence of beta-Cyclodextrins upon the Degradation of Carbofuran Derivatives under Alkaline Conditions" in the Journal of “Pesticides and Biofertilizers” to show that the cyclodextrins protect the carbamates increasing their half-life time in the presence of basic conditions This will be very helpful to understand carbofuran behaviour in the analytical, agro-environmental and food areas. We greatly appreciated the interaction with the editor and the editorial team; we were particularly well accompanied during the course of the revision process, since all various steps towards publication were short and without delay".

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Jesus Simal-Gandara

I would like to express my gratitude towards you process of article review and submission. I found this to be very fair and expedient. Your follow up has been excellent. I have many publications in national and international journal and your process has been one of the best so far. Keep up the great work.

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Douglas Miyazaki

We are grateful for this opportunity to provide a glowing recommendation to the Journal of Psychiatry and Psychotherapy. We found that the editorial team were very supportive, helpful, kept us abreast of timelines and over all very professional in nature. The peer review process was rigorous, efficient and constructive that really enhanced our article submission. The experience with this journal remains one of our best ever and we look forward to providing future submissions in the near future.

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Dr Griffith

I am very pleased to serve as EBM of the journal, I hope many years of my experience in stem cells can help the journal from one way or another. As we know, stem cells hold great potential for regenerative medicine, which are mostly used to promote the repair response of diseased, dysfunctional or injured tissue using stem cells or their derivatives. I think Stem Cell Research and Therapeutics International is a great platform to publish and share the understanding towards the biology and translational or clinical application of stem cells.

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Dr Tong Ming Liu

I would like to give my testimony in the support I have got by the peer review process and to support the editorial office where they were of asset to support young author like me to be encouraged to publish their work in your respected journal and globalize and share knowledge across the globe. I really give my great gratitude to your journal and the peer review including the editorial office.

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Husain Taha Radhi

I am delighted to publish our manuscript entitled "A Perspective on Cocaine Induced Stroke - Its Mechanisms and Management" in the Journal of Neuroscience and Neurological Surgery. The peer review process, support from the editorial office, and quality of the journal are excellent. The manuscripts published are of high quality and of excellent scientific value. I recommend this journal very much to colleagues.

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S Munshi

Dr.Tania Muñoz, My experience as researcher and author of a review article in The Journal Clinical Cardiology and Interventions has been very enriching and stimulating. The editorial team is excellent, performs its work with absolute responsibility and delivery. They are proactive, dynamic and receptive to all proposals. Supporting at all times the vast universe of authors who choose them as an option for publication. The team of review specialists, members of the editorial board, are brilliant professionals, with remarkable performance in medical research and scientific methodology. Together they form a frontline team that consolidates the JCCI as a magnificent option for the publication and review of high-level medical articles and broad collective interest. I am honored to be able to share my review article and open to receive all your comments.

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Tania Munoz

“The peer review process of JPMHC is quick and effective. Authors are benefited by good and professional reviewers with huge experience in the field of psychology and mental health. The support from the editorial office is very professional. People to contact to are friendly and happy to help and assist any query authors might have. Quality of the Journal is scientific and publishes ground-breaking research on mental health that is useful for other professionals in the field”.

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George Varvatsoulias

Dear editorial department: On behalf of our team, I hereby certify the reliability and superiority of the International Journal of Clinical Case Reports and Reviews in the peer review process, editorial support, and journal quality. Firstly, the peer review process of the International Journal of Clinical Case Reports and Reviews is rigorous, fair, transparent, fast, and of high quality. The editorial department invites experts from relevant fields as anonymous reviewers to review all submitted manuscripts. These experts have rich academic backgrounds and experience, and can accurately evaluate the academic quality, originality, and suitability of manuscripts. The editorial department is committed to ensuring the rigor of the peer review process, while also making every effort to ensure a fast review cycle to meet the needs of authors and the academic community. Secondly, the editorial team of the International Journal of Clinical Case Reports and Reviews is composed of a group of senior scholars and professionals with rich experience and professional knowledge in related fields. The editorial department is committed to assisting authors in improving their manuscripts, ensuring their academic accuracy, clarity, and completeness. Editors actively collaborate with authors, providing useful suggestions and feedback to promote the improvement and development of the manuscript. We believe that the support of the editorial department is one of the key factors in ensuring the quality of the journal. Finally, the International Journal of Clinical Case Reports and Reviews is renowned for its high- quality articles and strict academic standards. The editorial department is committed to publishing innovative and academically valuable research results to promote the development and progress of related fields. The International Journal of Clinical Case Reports and Reviews is reasonably priced and ensures excellent service and quality ratio, allowing authors to obtain high-level academic publishing opportunities in an affordable manner. I hereby solemnly declare that the International Journal of Clinical Case Reports and Reviews has a high level of credibility and superiority in terms of peer review process, editorial support, reasonable fees, and journal quality. Sincerely, Rui Tao.

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Rui Tao

Clinical Cardiology and Cardiovascular Interventions I testity the covering of the peer review process, support from the editorial office, and quality of the journal.

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Khurram Arshad

Clinical Cardiology and Cardiovascular Interventions, we deeply appreciate the interest shown in our work and its publication. It has been a true pleasure to collaborate with you. The peer review process, as well as the support provided by the editorial office, have been exceptional, and the quality of the journal is very high, which was a determining factor in our decision to publish with you.

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Gomez Barriga Maria Dolores

The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews journal clinically in the future time.

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Lin Shaw Chin

Clinical Cardiology and Cardiovascular Interventions, I would like to express my sincerest gratitude for the trust placed in our team for the publication in your journal. It has been a true pleasure to collaborate with you on this project. I am pleased to inform you that both the peer review process and the attention from the editorial coordination have been excellent. Your team has worked with dedication and professionalism to ensure that your publication meets the highest standards of quality. We are confident that this collaboration will result in mutual success, and we are eager to see the fruits of this shared effort.

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Maria Dolores Gomez Barriga

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, I hope this message finds you well. I want to express my utmost gratitude for your excellent work and for the dedication and speed in the publication process of my article titled "Navigating Innovation: Qualitative Insights on Using Technology for Health Education in Acute Coronary Syndrome Patients." I am very satisfied with the peer review process, the support from the editorial office, and the quality of the journal. I hope we can maintain our scientific relationship in the long term.

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Dr Maria Dolores Gomez Barriga

Dear Monica Gissare, - Editorial Coordinator of Nutrition and Food Processing. ¨My testimony with you is truly professional, with a positive response regarding the follow-up of the article and its review, you took into account my qualities and the importance of the topic¨.

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Dr Maria Regina Penchyna Nieto

Dear Dr. Jessica Magne, Editorial Coordinator 0f Clinical Cardiology and Cardiovascular Interventions, The review process for the article “The Handling of Anti-aggregants and Anticoagulants in the Oncologic Heart Patient Submitted to Surgery” was extremely rigorous and detailed. From the initial submission to the final acceptance, the editorial team at the “Journal of Clinical Cardiology and Cardiovascular Interventions” demonstrated a high level of professionalism and dedication. The reviewers provided constructive and detailed feedback, which was essential for improving the quality of our work. Communication was always clear and efficient, ensuring that all our questions were promptly addressed. The quality of the “Journal of Clinical Cardiology and Cardiovascular Interventions” is undeniable. It is a peer-reviewed, open-access publication dedicated exclusively to disseminating high-quality research in the field of clinical cardiology and cardiovascular interventions. The journal's impact factor is currently under evaluation, and it is indexed in reputable databases, which further reinforces its credibility and relevance in the scientific field. I highly recommend this journal to researchers looking for a reputable platform to publish their studies.

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Dr Marcelo Flavio Gomes Jardim Filho

Dear Editorial Coordinator of the Journal of Nutrition and Food Processing! "I would like to thank the Journal of Nutrition and Food Processing for including and publishing my article. The peer review process was very quick, movement and precise. The Editorial Board has done an extremely conscientious job with much help, valuable comments and advices. I find the journal very valuable from a professional point of view, thank you very much for allowing me to be part of it and I would like to participate in the future!”

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Zsuzsanna Bene

Dealing with The Journal of Neurology and Neurological Surgery was very smooth and comprehensive. The office staff took time to address my needs and the response from editors and the office was prompt and fair. I certainly hope to publish with this journal again.Their professionalism is apparent and more than satisfactory. Susan Weiner

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Dr Susan Weiner

My Testimonial Covering as fellowing: Lin-Show Chin. The peer reviewers process is quick and effective, the supports from editorial office is excellent, the quality of journal is high. I would like to collabroate with Internatioanl journal of Clinical Case Reports and Reviews.

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Lin-Show Chin

My experience publishing in Psychology and Mental Health Care was exceptional. The peer review process was rigorous and constructive, with reviewers providing valuable insights that helped enhance the quality of our work. The editorial team was highly supportive and responsive, making the submission process smooth and efficient. The journal's commitment to high standards and academic rigor makes it a respected platform for quality research. I am grateful for the opportunity to publish in such a reputable journal.

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Sonila Qirko

My experience publishing in International Journal of Clinical Case Reports and Reviews was exceptional. I Come forth to Provide a Testimonial Covering the Peer Review Process and the editorial office for the Professional and Impartial Evaluation of the Manuscript.

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Luiz Sellmann

I would like to offer my testimony in the support. I have received through the peer review process and support the editorial office where they are to support young authors like me, encourage them to publish their work in your esteemed journals, and globalize and share knowledge globally. I really appreciate your journal, peer review, and editorial office.

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Zhao Jia

Dear Agrippa Hilda- Editorial Coordinator of Journal of Neuroscience and Neurological Surgery, "The peer review process was very quick and of high quality, which can also be seen in the articles in the journal. The collaboration with the editorial office was very good."

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Thomas Urban

I would like to express my sincere gratitude for the support and efficiency provided by the editorial office throughout the publication process of my article, “Delayed Vulvar Metastases from Rectal Carcinoma: A Case Report.” I greatly appreciate the assistance and guidance I received from your team, which made the entire process smooth and efficient. The peer review process was thorough and constructive, contributing to the overall quality of the final article. I am very grateful for the high level of professionalism and commitment shown by the editorial staff, and I look forward to maintaining a long-term collaboration with the International Journal of Clinical Case Reports and Reviews.

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Cristina Berriozabal

To Dear Erin Aust, I would like to express my heartfelt appreciation for the opportunity to have my work published in this esteemed journal. The entire publication process was smooth and well-organized, and I am extremely satisfied with the final result. The Editorial Team demonstrated the utmost professionalism, providing prompt and insightful feedback throughout the review process. Their clear communication and constructive suggestions were invaluable in enhancing my manuscript, and their meticulous attention to detail and dedication to quality are truly commendable. Additionally, the support from the Editorial Office was exceptional. From the initial submission to the final publication, I was guided through every step of the process with great care and professionalism. The team's responsiveness and assistance made the entire experience both easy and stress-free. I am also deeply impressed by the quality and reputation of the journal. It is an honor to have my research featured in such a respected publication, and I am confident that it will make a meaningful contribution to the field.

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Dr Tewodros Kassahun Tarekegn

"I am grateful for the opportunity of contributing to [International Journal of Clinical Case Reports and Reviews] and for the rigorous review process that enhances the quality of research published in your esteemed journal. I sincerely appreciate the time and effort of your team who have dedicatedly helped me in improvising changes and modifying my manuscript. The insightful comments and constructive feedback provided have been invaluable in refining and strengthening my work".

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Dr Shweta Tiwari

I thank the ‘Journal of Clinical Research and Reports’ for accepting this article for publication. This is a rigorously peer reviewed journal which is on all major global scientific data bases. I note the review process was prompt, thorough and professionally critical. It gave us an insight into a number of important scientific/statistical issues. The review prompted us to review the relevant literature again and look at the limitations of the study. The peer reviewers were open, clear in the instructions and the editorial team was very prompt in their communication. This journal certainly publishes quality research articles. I would recommend the journal for any future publications.

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Dr Farooq Wandroo

Dear Jessica Magne, with gratitude for the joint work. Fast process of receiving and processing the submitted scientific materials in “Clinical Cardiology and Cardiovascular Interventions”. High level of competence of the editors with clear and correct recommendations and ideas for enriching the article.

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Dr Anyuta Ivanova

We found the peer review process quick and positive in its input. The support from the editorial officer has been very agile, always with the intention of improving the article and taking into account our subsequent corrections.

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Dr David Vinyes

My article, titled 'No Way Out of the Smartphone Epidemic Without Considering the Insights of Brain Research,' has been republished in the International Journal of Clinical Case Reports and Reviews. The review process was seamless and professional, with the editors being both friendly and supportive. I am deeply grateful for their efforts.

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Gertraud Teuchert-Noodt

To Dear Erin Aust – Editorial Coordinator of Journal of General Medicine and Clinical Practice! I declare that I am absolutely satisfied with your work carried out with great competence in following the manuscript during the various stages from its receipt, during the revision process to the final acceptance for publication. Thank Prof. Elvira Farina

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Dr Elvira Farina

Dear Jessica, and the super professional team of the ‘Clinical Cardiology and Cardiovascular Interventions’ I am sincerely grateful to the coordinated work of the journal team for the no problem with the submission of my manuscript: “Cardiometabolic Disorders in A Pregnant Woman with Severe Preeclampsia on the Background of Morbid Obesity (Case Report).” The review process by 5 experts was fast, and the comments were professional, which made it more specific and academic, and the process of publication and presentation of the article was excellent. I recommend that my colleagues publish articles in this journal, and I am interested in further scientific cooperation. Sincerely and best wishes, Dr. Oleg Golyanovskiy.

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Dr Oleg Golyanovski

Dear Ashley Rosa, Editorial Coordinator of the journal - Psychology and Mental Health Care. " The process of obtaining publication of my article in the Psychology and Mental Health Journal was positive in all areas. The peer review process resulted in a number of valuable comments, the editorial process was collaborative and timely, and the quality of this journal has been quickly noticed, resulting in alternative journals contacting me to publish with them." Warm regards, Susan Anne Smith, PhD. Australian Breastfeeding Association.

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Dr Susan Anne Smith

Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. I appreciate the journal (JCCI) editorial office support, the entire team leads were always ready to help, not only on technical front but also on thorough process. Also, I should thank dear reviewers’ attention to detail and creative approach to teach me and bring new insights by their comments. Surely, more discussions and introduction of other hemodynamic devices would provide better prevention and management of shock states. Your efforts and dedication in presenting educational materials in this journal are commendable. Best wishes from, Farahnaz Fallahian.

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Dr Farahnaz Fallahian

Dear Maria Emerson, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. I am delighted to have published our manuscript, "Acute Colonic Pseudo-Obstruction (ACPO): A rare but serious complication following caesarean section." I want to thank the editorial team, especially Maria Emerson, for their prompt review of the manuscript, quick responses to queries, and overall support. Yours sincerely Dr. Victor Olagundoye.

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Dr Victor Olagundoye

Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. Many thanks for publishing this manuscript after I lost confidence the editors were most helpful, more than other journals Best wishes from, Susan Anne Smith, PhD. Australian Breastfeeding Association.

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Dr Susan Anne Smith

Dear Agrippa Hilda, Editorial Coordinator, Journal of Neuroscience and Neurological Surgery. The entire process including article submission, review, revision, and publication was extremely easy. The journal editor was prompt and helpful, and the reviewers contributed to the quality of the paper. Thank you so much! Eric Nussbaum, MD

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Dr Eric S Nussbaum

Dr Hala Al Shaikh This is to acknowledge that the peer review process for the article ’ A Novel Gnrh1 Gene Mutation in Four Omani Male Siblings, Presentation and Management ’ sent to the International Journal of Clinical Case Reports and Reviews was quick and smooth. The editorial office was prompt with easy communication.

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Hala Al Shaikh

Dear Erin Aust, Editorial Coordinator, Journal of General Medicine and Clinical Practice. We are pleased to share our experience with the “Journal of General Medicine and Clinical Practice”, following the successful publication of our article. The peer review process was thorough and constructive, helping to improve the clarity and quality of the manuscript. We are especially thankful to Ms. Erin Aust, the Editorial Coordinator, for her prompt communication and continuous support throughout the process. Her professionalism ensured a smooth and efficient publication experience. The journal upholds high editorial standards, and we highly recommend it to fellow researchers seeking a credible platform for their work. Best wishes By, Dr. Rakhi Mishra.

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Dr Rakhi Mishra

Dear Jessica Magne, Editorial Coordinator, Clinical Cardiology and Cardiovascular Interventions, Auctores Publishing LLC. The peer review process of the journal of Clinical Cardiology and Cardiovascular Interventions was excellent and fast, as was the support of the editorial office and the quality of the journal. Kind regards Walter F. Riesen Prof. Dr. Dr. h.c. Walter F. Riesen.

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Dr Walter F Riesen

Dear Ashley Rosa, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews, Auctores Publishing LLC. Thank you for publishing our article, Exploring Clozapine's Efficacy in Managing Aggression: A Multiple Single-Case Study in Forensic Psychiatry in the international journal of clinical case reports and reviews. We found the peer review process very professional and efficient. The comments were constructive, and the whole process was efficient. On behalf of the co-authors, I would like to thank you for publishing this article. With regards, Dr. Jelle R. Lettinga.

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Dr Jelle Lettinga

Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, I would like to express my deep admiration for the exceptional professionalism demonstrated by your journal. I am thoroughly impressed by the speed of the editorial process, the substantive and insightful reviews, and the meticulous preparation of the manuscript for publication. Additionally, I greatly appreciate the courteous and immediate responses from your editorial office to all my inquiries. Best Regards, Dariusz Ziora

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Dariusz Ziora

Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation, Auctores Publishing LLC, We would like to thank the editorial team for the smooth and high-quality communication leading up to the publication of our article in the Journal of Neurodegeneration and Neurorehabilitation. The reviewers have extensive knowledge in the field, and their relevant questions helped to add value to our publication. Kind regards, Dr. Ravi Shrivastava.

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Dr Ravi Shrivastava

Dear Clarissa Eric, Editorial Coordinator, Journal of Clinical Case Reports and Studies, Auctores Publishing LLC, USA Office: +1-(302)-520-2644. I would like to express my sincere appreciation for the efficient and professional handling of my case report by the ‘Journal of Clinical Case Reports and Studies’. The peer review process was not only fast but also highly constructive—the reviewers’ comments were clear, relevant, and greatly helped me improve the quality and clarity of my manuscript. I also received excellent support from the editorial office throughout the process. Communication was smooth and timely, and I felt well guided at every stage, from submission to publication. The overall quality and rigor of the journal are truly commendable. I am pleased to have published my work with Journal of Clinical Case Reports and Studies, and I look forward to future opportunities for collaboration. Sincerely, Aline Tollet, UCLouvain.

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Dr Aline Tollet

Dear Ms. Mayra Duenas, Editorial Coordinator, International Journal of Clinical Case Reports and Reviews. “The International Journal of Clinical Case Reports and Reviews represented the “ideal house” to share with the research community a first experience with the use of the Simeox device for speech rehabilitation. High scientific reputation and attractive website communication were first determinants for the selection of this Journal, and the following submission process exceeded expectations: fast but highly professional peer review, great support by the editorial office, elegant graphic layout. Exactly what a dynamic research team - also composed by allied professionals - needs!" From, Chiara Beccaluva, PT - Italy.

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Dr Chiara Giuseppina Beccaluva

Dear Maria Emerson, Editorial Coordinator, we have deeply appreciated the professionalism demonstrated by the International Journal of Clinical Case Reports and Reviews. The reviewers have extensive knowledge of our field and have been very efficient and fast in supporting the process. I am really looking forward to further collaboration. Thanks. Best regards, Dr. Claudio Ligresti

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Dr Claudio Ligresti

Dear Chrystine Mejia, Editorial Coordinator, Journal of Neurodegeneration and Neurorehabilitation. “The peer review process was efficient and constructive, and the editorial office provided excellent communication and support throughout. The journal ensures scientific rigor and high editorial standards, while also offering a smooth and timely publication process. We sincerely appreciate the work of the editorial team in facilitating the dissemination of innovative approaches such as the Bonori Method.” Best regards, Dr. Matteo Bonori.

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Dr Matteo Bonori

I recommend without hesitation submitting relevant papers on medical decision making to the International Journal of Clinical Case Reports and Reviews. I am very grateful to the editorial staff. Maria Emerson was a pleasure to communicate with. The time from submission to publication was an extremely short 3 weeks. The editorial staff submitted the paper to three reviewers. Two of the reviewers commented positively on the value of publishing the paper. The editorial staff quickly recognized the third reviewer’s comments as an unjust attempt to reject the paper. I revised the paper as recommended by the first two reviewers.

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Edouard Kujawski

Dear Maria Emerson, Editorial Coordinator, Journal of Clinical Research and Reports. Thank you for publishing our case report: "Clinical Case of Effective Fetal Stem Cells Treatment in a Patient with Autism Spectrum Disorder" within the "Journal of Clinical Research and Reports" being submitted by the team of EmCell doctors from Kyiv, Ukraine. We much appreciate a professional and transparent peer-review process from Auctores. All research Doctors are so grateful to your Editorial Office and Auctores Publishing support! I amiably wish our article publication maintained a top quality of your International Scientific Journal. My best wishes for a prosperity of the Journal of Clinical Research and Reports. Hope our scientific relationship and cooperation will remain long lasting. Thank you very much indeed. Kind regards, Dr. Andriy Sinelnyk Cell Therapy Center EmCell

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Dr Andriy Sinelnyk

Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. It was truly a rewarding experience to work with the journal “Clinical Cardiology and Cardiovascular Interventions”. The peer review process was insightful and encouraging, helping us refine our work to a higher standard. The editorial office offered exceptional support with prompt and thoughtful communication. I highly value the journal’s role in promoting scientific advancement and am honored to be part of it. Best regards, Meng-Jou Lee, MD, Department of Anesthesiology, National Taiwan University Hospital.

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Dr Meng-JouLe

Dear Editorial Team, Journal-Clinical Cardiology and Cardiovascular Interventions, “Publishing my article with Clinical Cardiology and Cardiovascular Interventions has been a highly positive experience. The peer-review process was rigorous yet supportive, offering valuable feedback that strengthened my work. The editorial team demonstrated exceptional professionalism, prompt communication, and a genuine commitment to maintaining the highest scientific standards. I am very pleased with the publication quality and proud to be associated with such a reputable journal.” Warm regards, Dr. Mahmoud Kamal Moustafa Ahmed

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Mahmoud Kamal Moustafa Ahmed

Dear Maria Emerson, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews’, I appreciate the opportunity to publish my article with your journal. The editorial office provided clear communication during the submission and review process, and I found the overall experience professional and constructive. Best regards, Elena Salvatore.

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Dr Elena Salvatore

Dear Mayra Duenas, Editorial Coordinator of ‘International Journal of Clinical Case Reports and Reviews Herewith I confirm an optimal peer review process and a great support of the editorial office of the present journal

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Christoph Maurer

Dear Editorial Team, Clinical Cardiology and Cardiovascular Interventions. I am really grateful for the peers review; their feedback gave me the opportunity to reflect on the message and impact of my work and to ameliorate the article. The editors did a great job in addition by encouraging me to continue with the process of publishing.

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Baciulescu Laura

Dear Cecilia Lilly, Editorial Coordinator, Endocrinology and Disorders, Thank you so much for your quick response regarding reviewing and all process till publishing our manuscript entitled: Prevalence of Pre-Diabetes and its Associated Risk Factors Among Nile College Students, Sudan. Best regards, Dr Mamoun Magzoub.

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Dr Mamoun Magzoub